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Fig. 5 | BMC Urology

Fig. 5

From: MicroRNA-4429 suppresses proliferation of prostate cancer cells by targeting distal-less homeobox 1 and inactivating the Wnt/β-catenin pathway

Fig. 5

DLX1 blocks the antitumor function of miR-4429 in PCa cells. a DLX1 protein level in DU145 and PC-3 cells introduced with miR-4429 mimic and pcDNA DLX1 determined by western blot analysis (one-way ANOVA, P < 0.0001) (see original western blots in Additional file 1: Figure S5). b Proliferation of DU145 and PC-3 cells introduced with miR-4429 mimic and pcDNA DLX1 determined by CCK-8 assay (one-way ANOVA, P < 0.0001). c Expression of Axin2 and CD44 in DU145 and PC-3 cells introduced with miR-4429 mimic and PcDNA DLX1 determined by RT-qPCR (one-way ANOVA, P < 0.0001). d Number of colonies of DU145 and PC-3 cells introduced with miR-4429 mimic and PcDNA DLX1 determined by colony formation assay (one-way ANOVA, P < 0.0001). e Apoptosis in DU145 and PC-3 cells introduced with miR-4429 mimic and PcDNA DLX1 determined by flow cytometry (one-way ANOVA, P < 0.0001). f Invasion ability in DU145 and PC-3 cells introduced with miR-4429 mimic and PcDNA DLX1 determined by Transwell assay (one-way ANOVA, P < 0.0001). Data were expressed as mean ± SD from three independent experiments. *P < 0.05 was considered to show statistical significance

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BMC Urology

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