Fig. 1
From: Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma
CD276 transcript and protein expression. a Steady state mRNA amounts encoding CD276 were enumerated by qRT-PCR in four normal urothelial cells (NUCs) and four urothelial cancer cell lines (UCCLs). The graph shows the mean transcript levels ± standard deviations normalized to two housekeeping genes. b The total protein expression was investigated by immuno blotting of SDS-PAGEs. The mean expression of CD276 ± standard deviation of the four NUCs and four UCCLs is normalized to β-actin expression in each individual sample. UCCLs express significantly more CD276 transcripts (p < 0.006) and protein (p < 0.005) when compared to NUCs