Fig. 2From: Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinomaCell surface expression of CD276. The density of CD276 on cell surfaces was enumerated by flow cytometry on normal urothelial cells (NUCs) and urothelial cancer cell lines (UCCLs). a The representative NUC (HL19/17) shows a mean fluorescence intensity (MFI) of 2E03 and 91.8% of cells were gated positive for CD276 (solid histogram). b The representative tumor cell line HT1197 shows a MFI of 1.12E04 and 99.1% of cells were gated CD276 positive. Dotted histograms present isotype controls. The x-axis denotes the fluorescence intensity, the Y-axis the number of cells recorded as % of the maximumBack to article page