Table 2 Antibodies to human antigens and detection reagents for immunoblotting (IB), flow cytometry (FC), and immunohistochemistry (IHC)
From: Expression patterns of the immune checkpoint ligand CD276 in urothelial carcinoma
Target | Antibody/label | Species | Clone | Dilution | Source | Order # |
|---|---|---|---|---|---|---|
IB | ||||||
CD276 | IgG1 mAB | Mouse | 6A1 | 1:600 | abcam | ab105922 |
CD276 | serum | Rabbit | ø | 1:500 | abcam | ab226256 |
β actin | serum | Rabbit | ø | 1:1000 | abcam | ab8227 |
Mouse IgG | serum/HRP | Rabbit | ø | 1:2000 | Dako | P0260 |
Rabbit Ig | serum/HRP | Goat | ø | 1:2000 | Dako | 20034870 |
FC | ||||||
CD276 | IgG1 mAB/PE | Mouse | MIH42 | 1:20 | BioLegend | B7RP2 |
KLH | IgG1 mAB/PE | Mouse | X40 | 1:20 | BD | 345816 |
IHC | ||||||
CD276 | IgG1 mAB | Mouse | 6A1 | 1:300 | abcam | ab105922 |