Detection of AZF microdeletions and reproductive hormonal profile analysis of infertile sudanese men pursuing assisted reproductive approaches

Table 1 Sequence-tagged sites and primer sequences for Y chromosome microdeletion analysis

Region	STS		Sequence 5′– 3'	Size bp	References
Yp	sY14	Forward	GAATATTCCCGCTCTCCGGA	495	[9]
		Reverse	GCTGGTGCTCCATTCTTGAG
AZFa	sY81	Forward	AGGCACTGGTCAGAATGAAG	209	[8]
		Reverse	AATGGAAAATACAGCTCCCC
	sY84	Forward	AGAAGGGTCTGAAAGCAGGT	326	[9]
		Reverse	GCCTACTACCTGGAGGCTTC
	sY86	Forward	GTGACACACAGACTATGCTTC	320	[9]
		Reverse	ACACACAGAGGGACAACCCT
AZFb	sY127	Forward	GGCTCACAAACGAAAAGAAA	274	[9]
		Reverse	CTGCAGGCAGTAATAAGGGA
	sY128	Forward	GGATGAGACATTTTTGTGGG	384	[10]
		Reverse	AGCCCAATGTAAACTGGACA
AZFc	sY239	Forward	CATTCATCTTCCCTTTTGAAGG	201	[8]
		Reverse	ATGCAAGTCGCAGGAAATCT
	sY254	Forward	GGGTGTTACCAGAAGGCAAA	370	[9]
		Reverse	GAACCGTATCTACCAAAGCAGC
	sY255	Forward	GTTACAGGATTCGGCGTGAT3	126	[9]
		Reverse	CTCGTCATGTGCAGCCAC
AZFd	sY152	Forward	AAGACAGTCTGCCATGTTTCA	125	[11]
		Reverse	ACAGGAGGGTACTTAGCAGT
	sY153	Forward	GCATCCTCATTTTATGTCCA	139	[8]
		Reverse	CAACCCAAAAGCACTGAGTA3

ISSN: 1471-2490