Fig. 5

Gemcitabine enhanced radiation response under normoxic and hypoxic conditions in the DU145 cells. a Gemcitabine (8 nM, 24 h) enhanced the radiosensitivity of normoxic DU145 cells, the SER was 1.27 and at 4 Gy gemcitabine significantly reduced surviving fraction compared to radiation alone. b Under hypoxia the SER of gemcitabine was 1.09 and at 4 Gy gemcitabine significantly reduced surviving fraction compared to radiation alone. c In the PC3 cell line, gemcitabine did not enhance the radiosensitivity of normoxic or d hypoxic cells. The data points represent the mean ± SEM of 2–4 values taken from each biological repeat, within each biological repeat there were 6 intra-assay replicates. Statistical analysis was performed using a paired t-test (ns = not significant, * indicates p < 0.05). SERs were calculated at SF10% and statistical analysis was performed using the F-test