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Fig. 3 | BMC Urology

Fig. 3

From: Metformin exerts an antitumor effect by inhibiting bladder cancer cell migration and growth, and promoting apoptosis through the PI3K/AKT/mTOR pathway

Fig. 3

In vitro, cell apoptosis was promoted by different of concentrations of metformin. A T24 cell apoptosis as detected using flow cytometry. The images show the number of Q2 + Q4 as apoptotic cells. B Western blotting detection of the levels of cleaved-caspase 3 and cleave-PARP in T24 cells. Full length blots/gels are presented in Additional file 1: Figure 3B. The loading control comprised GAPDH. Full length blots/gels are presented in Additional file 1: Figure 3B, 4A. C 5637 cell apoptosis as detected using flow cytometry. The images show the number of Q2 + Q4 as apoptotic cells. D Western blotting detection of the levels of cleaved-caspase 3 and cleave-PARP in 5637 cells. Full length blots/gels are presented in Additional file 1: Figure 3D. The loading control comprised GAPDH. Full length blots/gels are presented in Additional file 1: Figure 3D, 4B. The representative column diagrams showing results of relative protein expression. In the graphs, the results are shown as the mean ± the standard deviation (SD) from three independent experiments and were compared with the values for the control (*P < 0.05, **P < 0.01). PARP, poly(ADP-ribose) polymerase GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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BMC Urology

ISSN: 1471-2490

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