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Fig. 4 | BMC Urology

Fig. 4

From: Metformin exerts an antitumor effect by inhibiting bladder cancer cell migration and growth, and promoting apoptosis through the PI3K/AKT/mTOR pathway

Fig. 4

The different of concentrations of Metformin inhibited PI3K/AKT/mTOR pathway activation in vitro. A Western blotting detection of PI3K/AKT/mTOR and p-PI3K/p-AKT/p-mTOR protein levels in T24 cells. Full length blots/gels are presented in Additional file 1: Figure 4A. The loading control comprised GAPDH.  Full length blots/gels are presented in Additional file 1: Figure 3B, 4A. The graph shows the relative protein levels. B Western blotting detection of PI3K/AKT/mTOR and p-PI3K/p-AKT/p-mTOR protein levels in 5637 cells.  Full length blots/gels are presented in Additional file 1: Figure 4B. The loading control comprised GAPDH. Full length blots/gels are presented in Additional file 1: Figure 3D, 4B. The graph shows the relative protein levels. Data are shown as the mean ± SD of three independently performed experiments (*P < 0.05, **P < 0.01). PI3K, phosphatidylinositol-4,5-bisphosphate 3-kinase; p-PI3K, phosphorylated PI3K; Akt, protein kinase B; p-Akt, phosphorylated Akt; mTOR, mechanistic target of rapamycin; p-mTOR, phosphorylated mTOR; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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