Skip to main content
Fig. 3 | BMC Urology

Fig. 3

From: Upregulation of mir-1199-5p is associated with reduced type 2 5-α reductase expression in benign prostatic hyperplasia

Fig. 3

miR-1199-5p regulating the expression of SRD5A2. (A)The RT-qPCR results showed that SRD5A2 mRNA was significantly downregulated after transfection with miR-1199-5p mimics in RWPE-1 cells. (B-C) The Western Blotting results showed that miR-1199-5p mimics decreased the expression of SRD5A2 in RWPE-1 cells and miR-1199-5p inhibitors rescued the expression of SRD5A2 in BPH-1 cells. (D) Schematic representation of the mature miR-1199-5p sequence, putative miR‐1199-5p target site in the 3’UTR of SRD5A2 mRNA. (E) Overexpression of miR‐1199-5p markedly decreased the relative luciferase activity in the WT 3’UTR of SRD5A2 mRNA, while the mutated 3’UTR of SRD5A2 was insensitive to miR‐1199-5p overexpression. (F) The apoptosis of BPH-1 treated with finasteride (100 µM)/DMSO after transfection with miR-1199-5p inhibitor/ inhibitor-NC. (G) The apoptosis of RWPE-1 treated with finasteride (100 µM)/DMSO after transfection with miR-1199-5p mimics/mimics-NC. ***P<0.001; SRD5A2: 5-α reductase type 2; BPH-1: immortalized benign prostatic hyperplasia epithelial cells; RWPE-1: normal prostate epithelial cell line. The original blots/gels are presented in Additional file : Fig S3-4

Back to article page