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Fig. 2 | BMC Urology

Fig. 2

From: NEAT1 promotes the malignant development of bladder cancer by regulating the miR-101/VEGF-C pathway in vitro and in vivo

Fig. 2

Effects of the NEAT1/miR-101 pathway on BC cells. A The putative binding sites of NEAT1 with miR-101 are listed. The results of the luciferase assay indicated that overexpressed miR-101 significantly decreased the activity of the wild-type NEAT1 3′-UTR reporter but did not influence the activity of the mutant NEAT1 3′-UTR reporter. **p < 0.01 miR-101 group versus NC group. B After 24 h, the proliferation of the cells with enforced NEAT1 expression and a miR-101 inhibitor was significantly higher than that in the NEAT1 group in both T24 and 5637 cells. **p < 0.01 miR-101 inhibitor + NEAT1 group versus NEAT1 group. The proliferation of the cells with enforced NEAT1 expression and a miR-101 mimic was significantly lower than that in the NEAT1 group in both T24 and 5637 cells. **p < 0.01 miR-101 mimic + NEAT1 group versus NEAT1 group. C, D The quantitative results of the migration and invasion assays showed that the migration and invasion of the cells with enforced NEAT1 expression and a miR-101 inhibitor were significantly higher than those in the NEAT1 group both in T24 and 5637 cells. **p < 0.01 miR-101 inhibitor + NEAT1 group versus NEAT1 group. The migration and invasion of the cells with enforced NEAT1 expression and a miR-101 mimic were significantly lower than those in the NEAT1 group in both T24 and 5637 cells. **p < 0.01 miR-101 mimic + NEAT1 group versus NEAT1 group. E The expression of miR-101 mRNA was significantly increased after transfection with NEAT1 and a miR-101 mimic and decreased after transfection with NEAT1 and a miR-101 inhibitor. **p < 0.01 miR-101 mimic + NEAT1/miR-101 inhibitor + NEAT1 group versus NEAT1 group. The expression of VEGF-C mRNA was significantly decreased after transfection with NEAT1 and a miR-101 mimic and increased after transfection with NEAT1 and a miR-101 inhibitor. **p < 0.01 miR-101 mimic + NEAT1/miR-101 inhibitor + NEAT1 group versus NEAT1 group. F. The VEGF-C levels of the cells were significantly decreased after transfection with NEAT1 and a miR-101 mimic and increased after transfection with NEAT1 and a miR-101 inhibitor (Additional file 2: Figure S2). **p < 0.01 miR-101 mimic + NEAT1/miR-101 inhibitor + NEAT1 group versus NEAT1 group

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