Skip to main content
Figure 6 | BMC Urology

Figure 6

From: Substance P induces localization of MIF/α1-inhibitor-3 complexes to umbrella cells via paracellular transit through the urothelium in the rat bladder

Figure 6

Substance P-induced release of MIF/A1-I3 complexes in the intraluminal fluid. A) MIF WB of serum, urine collected before treatment and intraluminal fluid of saline treated vs SP-treated rats demonstrated differences in the forms of MIF found. Serum only contained high-molecular weight MIF complexes of 170 and 130-kDa apparent molecular weight. No 12-kDa MIF was detected in the serum. In pre-treatment urine, however, only 170-kDa MIF/A1-I3 (as verified by A1-I3 WB; data not shown) and 12-kDa MIF bands but no 130-kDa were observed. B) In the intraluminal fluid (ILF), 170 and 130-kDa bands, corresponding to MIF/A1-I3 complexes [10] were noted along with 12-kDa MIF. SP treatment increased both 170 and 130 kDa MIF/A1-I3 amounts in the ILF (Figure B).

Back to article page

BMC Urology

ISSN: 1471-2490

Contact us