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Fig. 2 | BMC Urology

Fig. 2

From: Anti-tumor effects of a recombinant anti-prostate specific membrane antigen immunotoxin against prostate cancer cells

Fig. 2

Detection of immunocasp-3 protein secreted by genetically modified Jurkat cells. a: Genomic DNA was isolated from the immunocasp-3-modified Jurkat cell clones and analyzed by PCR: J591 (lane 1); revcaspase-3 (lane 2); β-actin (lane 3), respectively. b: Western blot analysis of the concentrated cell culture medium obtained from Jurkat cells stably transfected with the immunocasp-3 gene. Blots were probed with the anti-caspase-3 antibody. c: Growth and proliferation characteristics of Jurkat cells transfected with immunocasp-3 or unmodified Jurkat cells

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