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Fig. 1 | BMC Urology

Fig. 1

From: Distinct effects of Fgf7 and Fgf10 on the terminal differentiation of murine bladder urothelium revealed using an organoid culture system

Fig. 1

Differentiation status of bladder organoid derived from adult mice. (A) Schematic representation of the experiment. Bladders were taken out from adult (8–10 weeks of age) or neonatal mice (postnatal day 1). Adult bladders were further processed for epithelial isolation for following organoid culture. mRNA were isolated from the whole extract of adult (adult bladder tissues; AdBT) bladders, neonatal (neonatal bladder tissues; NeoBT) bladders, the isolated epithelia (adult bladder epithelium; AdBE), and organoid cells cultured for 1 week (adult bladder organoids; AdBO) for qPCR. A representative image of AdBO is shown. Scale bar: 500 μm. (B) Expression levels of the differentiated cell markers (Krt20, Upk1a, Upk2, Upk3a) and immature cell markers (Krt5, Krt14, Trp63) in AdBT, AdBE, NeoBT, and AdBO as values adjusted with Gapdh levels are graphed by qPCR, respectively. Values represent the average of three independent experiments and error bars denote standard error of the mean. (C) Immunofluorescence of murine bladder organoids for staining with CK5 shown with Hoechst. Scale bar: 50 μm

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BMC Urology

ISSN: 1471-2490

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