Fig. 5

Expression patterns of Fgf7 and Fgf10 mRNA in murine bladder tissues. (A) Experimental protocol. The adult murine bladder specimens were sectioned at 5 mm and three serial sections were used for H&E staining, in-situ hybridization (ISH) with sense probes, or ISH with antisense probes, and immunofluorescence for CK5 and UPK3 staining. (B) Representative images for Fgf7 (top) and Fgf10 (bottom) analyses are shown. Histological image (H&E), ISH images with sense and anti-sense probes are shown from left to right. The magnified views from the dashed rectangles are shown in the rightmost column. Arrowheads and dotted arrowheads indicate signals of antisense at epithelial and stroma layer, respectively. Scale bar: 25 μm. (C) Relative expression levels of Fgf7 and Fgf10 in AdBT and AdBE by qPCR. **p < 0.01. (D) Other representative images obtained from different individual from B) for Fgf7 analysis are shown. ISH images with sense and anti-sense probes (upper left and right) and Histological images for H&E (lower left) and complementary staining with CK5 and UPK3 (lower right) are shown. Scale bar: 25 μm