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Fig. 3 | BMC Urology

Fig. 3

From: MicroRNA-148a-3p in pericyte-derived extracellular vesicles improves erectile function in diabetic mice by promoting cavernous neurovascular regeneration

Fig. 3

MCPs-EVs decreased apoptosis and increased proliferation of MCECs through miR-148a-3p under high-glucose (HG) conditions. a TUNEL (green) immunostaining in MCECs treated with PBS, MCPs-EVs-reagent control (MCPs-EVs-RC, 1 μg/mL), MCPs-EVs-miR-148a-3p inhibitor (MCPs-EVs-miR-148a-3p-i, 1 μg/mL) under normal-glucose (NG) and HG conditions for 3 days. Scale bar = 100 μm. b Number of apoptotic cells were quantified by ImageJ and the results are presented as mean ± SEM (n = 4). c PH3 (red) immunostaining in MCECs with the same treatment conditions as for TUNEL immunostaining. Nuclear were labeled with DAPI (blue). d Number of PH3-positive cells were quantified by ImageJ and the results are presented as mean ± SEM (n = 4). ***p < 0.001. TUNEL, terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling; MCPs, mouse corpus cavernous pericyte; MCECs, mouse cavernous endothelial cells; PH3, phospho-Histone H3; ns, not significant; DAPI, 4.6-diamidino-2-phenylindole

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BMC Urology

ISSN: 1471-2490

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