Fig. 1
From: USP54 is a potential therapeutic target in castration-resistant prostate cancer
USP54 is a possible therapeutic target in CRPC cells. A The viabilities of LNCaP and LNCaP-AI cells cultured in 10% (v/v) CS-FBS-supplemented medium were measured using the CCK-8 assay at the indicated times. B, C Colony formation by LNCaP and LNCaP-AI cells cultured in the same medium; the colony numbers are shown. D Expression of indicated protein in LNCaP and LNCaP_AI by Western blotting; β-actin was used as reference gene. E Expression of AR-response genes by LNCaP and LNCaP-AI cells; the heat map indicates differential expression levels. G Expression of USP proteins by LNCaP and LNCaP-AI cells; the heat map indicates differential expression levels. F Upregulated DUBs, as revealed by fold changes and –log10 padjust values. H The levels of mRNA encoding USP54, as measured by qPCR (t-test; error bars represent SDs; ***P < 0.001). I Expression of indicated protein in LNCaP and LNCaP_AI by Western blotting; vinculin was used as reference gene