Fig. 3From: USP54 is a potential therapeutic target in castration-resistant prostate cancerUSP54 knockdown impairs CRPC cell proliferation in vitro. A qPCR confirmed that the shRNA provided efficient knockdown. B WB confirmed that he shRNA provided efficient knockdown. C, D The viabilities of PC3 and 22Rv1 cells expressing shRNAs that target a control sequence or USP54, measured using the CCK-8 assay at the indicated times. E, F Colony formation assays of PC3 and 22Rv1 cells transfected with the indicated shRNAs; the colony numbers are shown. (t-test; error bars represent SDs; **P < 0.01; ***P < 0.001)Back to article page