Fig. 4
From: USP54 is a potential therapeutic target in castration-resistant prostate cancer
USP54 downregulated impedes CRPC cell growth in vivo. A PC3 cells (5 × 105) expressing shRNA targeting a control sequence and USP54 were suspended in Matrigel (1:1 v/v) and subcutaneously implanted into 6-week-old nude mice (n = 6); the mice were sacrificed after 35 days, and the volumes of xenograft tumors were determined. B The weights of xenografts in each group. Error bars: means ± SDs (Mann–Whitney test; n = 6; ***P < 0.001). C Kaplan–Meier analysis of tumor onset times (log-rank test; ***P < 0.001). D Hematoxylin–eosin (HE) and Ki-67 immunohistochemical staining of tumor xenografts (scale bar: 100 μm). E The numbers of cells expressing Ki-67 (Mann–Whitney test; **P < 0.01)