Fig. 6
Correlation analysis and validation of FRGs. (a) Correlation analysis between four DEGs and SLC7A11 and GPX4. (b) Gene co-expression network analysis of TMEFF2, SLC7A11, and GPX4. (c) The expression of SLC7A11 was suppressed by downregulating TMEFF2 in LNCaP and VCaP cells but not in PC3 and C4-2 cells. (d) The cell growth of LNCaP, VCaP, PC3 and C4-2 cells with knockdown of TMEFF2 was examined via a CCK8 assay. Three independent experiments were performed with triplicate wells. Representative experiments and images are shown.(e) The relative Fe2+ concentration of LNCaP, VCaP, PC3 and C4-2 cells with downregulation of TMEFF2 was detected. (f) After knockdown of TMEFF2 and enzalutamide treatment, the expression of SLC7A11, TMEFF2, and androgen receptor (AR) were detected by western blot. (g) The mRNA expression of TMEFF2 in normal tissue and prostate cancer tissue (including localized prostate tissue and CRPC tissue) was obtained from the GSE35988 dataset in the GEO database. The statistical significance of differences was calculated using an independent-sample t test. *p < 0.05, **p < 0.01