Table 1 Relative sample amount for which methylation can be determined as function of quantity and age
From: A tissue biopsy-based epigenetic multiplex PCR assay for prostate cancer detection
tissue biopsy volume | sample age | # samples (% correctly analyzable) | sufficient input DNA | methylated copies of | ||
|---|---|---|---|---|---|---|
GSTP1 | APC | RASSF1 | ||||
10 μm | > = 5 years | 45 (60%) | 67% | 81% | 81% | 48% |
<= 1 year | 53 (72%) | 87% | 80% | 86% | 84% | |
20 μm | > = 5 years | 45 (73%) | 78% | 90% | 93% | 81% |
<= 1 year | 53 (92%) | 100% | 91% | 94% | 93% | |
40 μm | > = 5 years | 45 (82%) | 93% | 86% | 88% | 83% |
<= 1 year | 53 (92%) | 100% | 96% | 90% | 93% | |