Figure 1

Comparison of culture mediums and supplements for MB49 cell cultures. The combination for an optimal SFM is RPMI1640+ EGF(E) + FGF-b(F) + LIF(L) + B27(B) + BSA. (A) SFM with the combination of all four growth factors E + F + L + B together provides a robust synergistic effect on MB49 cell morphology. The medium containing just one or two growth factors displays small sphere morphology. (B) The cell proliferation curve shows that the combination of E + F + L + B contains the highest absorbance value after using the Cell Counting Kit-8(CCK-8). (C) The largest numbers of colony-forming units is the combination of E + F + L + B. (D) The highest sphere-formation efficiency is the combination of E + F + L + B.