Figure 2

Morphology of MCSCs under serum-free medium and medium containing 10% fetal bovine serum (FBS). (A) Representative example of a CSC sphere formation originating from a single MB49 cell under an optimal serum-free culture medium. (B) MCSCs generated secondary passages. (C) MCSCs are differentiated and adherent to the culture dish when cultured in medium containing 10% FBS. (d = day; P = passage).