Table 1 Cell culture medium and supplements
From: A modified method for isolation of bladder cancer stem cells from a MB49 murine cell line
Reagent | Suppliers | Concentration |
|---|---|---|
EGF | Peprotech, Rocky Hill, NJ, USA | 20 ng/ml |
FGF-b | Peprotech | 20 ng/ml |
LIF | eBioscience, San Diego, CA, USA | 20 ng/ml |
B27 | Invitrogen, Grand Island, NY, USA | 20 μl/ml |
BSA | Thermo Scientific HyClone, Logan, UT, USA | 4 mg/ml |
RPMI1640 | Thermo Scientific HyClone | 1× |
DMEM/F12 | Thermo Scientific HyClone | 1× |