Table 3 Primers of selected genes
From: A modified method for isolation of bladder cancer stem cells from a MB49 murine cell line
Gene name | Primers (forward/reverse) | Base pairs of product |
|---|---|---|
CD133 | F: 5’-CGGGATCCGAAAAACTGATCTGT-3’ | 615 bp |
R: 5’-CCGCTCGAGTTACCTAGTTACTCTCTCC-3’ | ||
CD44 | F: 5’-CCCTGCTACCAGAGACCAAGAC-3’ | 401 bp |
R; 5’-GCAGGTTCCTTGTCTCATCAGC-3’ | ||
NANOG | F: 5’-CAGCTGTGTGTACTCAATGATAGATTT-3’ | 179 bp |
R: 5’-ACACCATTGCTATTCTTCGGCCAGTTG-3’ | ||
OCT4 | F: 5’-TCAGCCAAACGACCATCTGC-3’ | 205 bp |
R: 5’TTCTCCAGGTTGCCTCTCAC-3’ | ||
GAPDH | F: 5’-CCATGGAGAAGGCTGGGG-3’ | 198 bp |
R: 5’-CAAAGTTGTCATCCATGACC-3’ |