Table 1 Oligonucleotides used as primers and radiolabelled probes for HPV type-specific and consensus PCR.
Oligonucleotide | Nucleotide sequence | Localization in HPV genome |
|---|---|---|
TS-HPV6-1 | +5'-TAGTGGGCCTATGGCTCGTC-3' | E5: 4671–4690 |
TS-HPV6-2 | -5'-TCCATTAGCCTCCACGGGTG-3' | E5:4931–4950 |
TS-HPV6 probe | +5'-CATTAACGCAGGGGCGCCTGAAATTGTGCC-3' | E5: 4761–4790 |
TS-HPV11-1 | +5'-GGAATACATGCGCCATGTGG-3' | L1: 6841–6860 |
TS-HPV11-2 | -5'-CGAGCAGACGTCCGTCCTCG-3' | L1: 7181–7200 |
TS-HPV11 probe | +5'-CGCCTCCACCAAATGGTACACTGGAGGATA-3' | L1: 6977–7006 |
TS-HPV16-1 | +5'-TGCTAGTGCTTATGCAGCAA-3' | L1: 6028–6047 |
TS-HPV16-2 | -5'-ATTTACTGCAACATTGGTAC-3' | L1: 6160–6179 |
TS-HPV16 probe | +5'-CAAACCACCTATAGGGGAACACTGGGGCA-3' | L1: 6117–6146 |
TS-HPV18-1 | +5'-AAGGATGCTGCACCGGCTGA-3' | L1: 6903–6922 |
TS-HPV18-2 | -5'-CACGCACACGCTTGGCAGGT-3' | L1: 7100–7119 |
TS-HPV18 probe | +5'-TGGTTCAGGCTGGATTGCGTCGCAAGCCCA-3' | L1: 7021–7050 |
MY11 | +5'-GCMCAGGGWCATAAYAATGG-3' (W = A+T, Y = C+T; M = A+C) | L1: 6582–6601 |
MY09 | -5'-CGTCCMARRGGAWACTGATC-3' (W = A+T; R = A+G; M = A+C) | L1: 7033–7014 |
Consensus probe: | ||
MY18 | +5'-CTGTTGTTGATACTACACGCAGTAC-3' | L1 |
MY46 | +5'-CTGTGGTAGATACCACWCGCAGTAC-3' | L1 |
MY57 | +5'-CTGTGGTAGATACCACACGTAGTA-3' | L1 |
WD147 | +5'-CTGTAGTGGACACTACCCGCAGTAC-3' | L1 |