Figure 2
A. RT-PCR of HIF-1α. RT-PCR was performed as described in Materials and Methods and shows the presence of HIF-1α (upper panel) and β-actin as internal control (lower panel). Lane 1, normal prostate (PrEC). Lanes 2 and 3, androgen-independent prostate cell lines PC3 and DU145, respectively. Lane 4, LNCaP-R control. Lane 5, LNCaP-R treated 5 weeks in 1 μM Casodex. Lane 6, LNCaP-UR control. Lane 7, LNCaP-UR treated 5 weeks in 1 μM Casodex. B. Quantitative real time PCR for HIF-1α expression was perfomed as described in materials and methods. CT values was determined using the formula ΔΔCT = [ΔCT(sampleX)] - [ΔCT(calibratorsample)] and ΔCT = [CT(targetgene)] - [CT(referencegene)]. The graph was drawn using Graph Pad Prism 3.0.