Figure 8

RT-PCR of HIF-1 induced genes. LNCaP cells were treated with 1μM Casodex for 5 weeks. RNA was isolated from both treated and untreated cells as described in Materials and Methods. Expression of VEGF isoforms (panel A), Glut-1 (panel B), CA-IX (panel C), CA-XII (panel D) and β-Actin (panel E) were examined by RT-PCR. In each panel, the cell lines shown are PC-3 (lane 2), DU-145 (lane 3) LNCaP-RC (lane 4), LNCaP-RT (lane 5), LNCaP-URC (lane 6) and LNCaP-URT (lane 7). The 100 bp DNA ladder (lane1) is included as a size marker. PCR amplification was performed as described under Materials and Methods.