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Table 1 Comparison of ELISA and Western blotting in determining MIF levels

From: Substance P induces localization of MIF/α1-inhibitor-3 complexes to umbrella cells via paracellular transit through the urothelium in the rat bladder

 

Chemicon ELISA

Western Blotting

Bladder MIF (ng/mg protein)

Saline (n = 5)

694 ± 39.9

12870 ± 1150

Substance P (n = 5)

398 ± 56.7 **

8765 ± 726.8 *

ILF MIF (ng/ml)

Saline (n = 5)

2.5 ± 0.8

2821 ± 635.2

Substance P (n = 5)

19.0 ± 2.4 ***

4338 ± 281.7 *

  1. * = p < 0.05; ** = p < 0.01; *** = p < 0.001
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BMC Urology

ISSN: 1471-2490

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