TRPM8 immunoreactivity in human urinary bladder and dental pulp. TRPM8 urothelial staining and suburothelial fibres (arrowed) in urinary bladder (a), Scale bar = 100 μm. TRPM8-immunoreactive axon (A)(b) and myelin (M)(b-c) staining in suburothelium, Scale bar = 50 μm. Similar, TRPM8-immunoreactive axon (A) and myelin (M) staining in dental pulp (d), Scale bar = 100 μm. TRPM8-immunoreactive nerve fibres in suburothelium (e) are abolished after pre-incubation with excess peptide with TRPM8 antibody at a dilution of 1:1500 (f), Scale bar = 100 μm. Nuclei counterstained with neutral red.