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Figure 2 | BMC Urology

Figure 2

From: miR-221 facilitates the TGFbeta1-induced epithelial-mesenchymal transition in human bladder cancer cells by targeting STMN1

Figure 2

miR-221 negatively regulates STMN1 expression. (A) Luciferase reporter assay with co-transfection of wild-type or mutant STMN1 and miR-221 mimics or miR-221 inhibitor or negative–control or inhibitor negative-control or blank control in H9c2 cells. (B) qRT-PCR analyses were performed to examine the effects of miR-221 on expression of STMN1. (C) Western blotting was performed to determine effects of miR-221 on expression of STMN1 protein in T24 and RT4 cells. Error bars represent ± S.E. and *p < 0.01 versus control.

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