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Table 2 Dilutions of external DNA standards used in the real-time PCR assays

From: Altered PCA3 and TMPRSS2-ERG expression in histologically benign regions of cancerous prostates: a systematic, quantitative mRNA analysis in five prostates

 

Range (molecules per mL of template)

 

Target RNA

Lowest concentration

Highest concentration

Total number of points on standard curve

KLK3

2.5 × 103

2 × 1011

8

PCA3

1.3 × 103

2.5 × 1011

7

TMPRSS2-ERG III

5 × 103

5 × 107

4

TMPRSS2-ERG VI

2 × 104

2 × 108

4