Fig. 3
From: Regulation of urinary bladder function by protein kinase C in physiology and pathophysiology
Low and high levels of PKC stimulation by PDBu differentially affect DSM tone and spontaneous contractions. a Representative isolated muscle strips showing the effect of low (20nM) PDBu stimulation on sham (upper trace) and PBOO (lower trace) DSM strips from rabbits. Summary data is presented in c. b Representative isolated muscle strips showing the effect of high (100 nM) PDBu stimulation on sham (upper trace) and PBOO (lower trace). Summary data is presented in d. Low PDBu stimulation caused a significant inhibition of spontaneous contraction amplitude (SCA) of the sham muscle strips but only a minor effect in PBOO strips, however low PDBu caused a significant increase in basal DSM tone in the PBOO strips. High PDBu stimulation caused a significant increase in basal DSM tone for both sham and PBOO, however, there was a qualitative difference in the profile of the contractions. The force increase in the sham bladders displayed a biphasic effect whereas an increase in PBOO muscle strips tended to be more linear. High PDBu also significantly reduced the amplitude of spontaneous contractions for both sham and PBOO muscle strips