Fig. 2

The effect of signaling inhibitors on stretch-induced JNK1 activation. a HBSMCs were pre-incubated with 10 μg/ml lipofectamine and the RhoA inhibitor C3 exoenzyme (upper blots), the Rho kinase inhibitor Y-27632 (middle blots), or the JNK1 inhibitor SP600125 (bottom blots) at the indicated concentrations and the cells were then subjected (+), or not (−), to uni-axial stretch with 15 % elongation for 30 min. The activity of JNK1 was measured in the same manner as in Fig. 1. b Quantification of the effect of the inhibitors on JNK1 activation. Stretch induced JNK1 activity was quantified by densitometry. JNK1 activity under the conditions of pre-incubation with 5 μg/ml C3 exoenzyme, 1 μM Y-27632 or 10 μM SP600125 are indicated in the figure. Data were normalized by the total amount of JNK1 protein and the intensity of non-stretched, non-treated cells was defined as 1.0. Data are indicated as means ± SEM (n = 6). An * indicates p < 0.05