Fig. 4

Testis ultrastructural observations by SEM. a An intricate three-dimensional network and each seminiferous tubule with large fenestrae and well spaced cells were observed in the testis of control mice. b-d At 2 weeks, distinct globoid particles were observed between the interstitial tissue and the seminiferous tubules in the testis after 2 week in group C (Fig. 4b and c; red arrows). In addition, the seminiferous tubules showed a compact, fibrous appearance with absence of fenestrae, but with a larger number of cellular debris, and microcalcification surrounded by globoid NPs within the seminiferous tubules were obviously observed in affected tubules in group C (Fig. 4c-d; yellow arrow). e At 4 weeks, the epithelium height diminished and no spermatozoa in the lumen could be witnessed except microcalcification filled in the seminiferous tubule only in group C (Fig. 4e; white arrows)