Fig. 4

miR-221-5p regulates MAPK/ERK signaling pathways and affects EMT features in human prostate cancer cells. a Overexpression of miR-221-5p enhances MAPK/ERK signaling pathways. After serum starvation for 16 h, PC3 cells with overexpression of miR-221-5p or control were stimulated with 10% FBS for 20 min and the cells were harvested for immunoblotting. b Silencing of miR-221-5p inhibits MAPK/ERK signaling pathways. PC3 stably cells of silencing miR-221-5p or control were serum-starved for 16 h, and cells were stimulated with 10% FBS for 20 min. Western blot analysis of MAPK/ERK signaling pathways proteins in the cell samples. c, d miR-221-5p promotes EMT features and regulates the expression of mesenchymal marker vimentin and epithelial marker E-cadherin