Fig. 3

Fluorescence in-situ hybridization of the QC-TMA, with DNA probes detecting PTEN (orange), WAPAL (green), FAS (aqua), and CEP 10 (red). a Cells representing no PTEN deletion. b Cells showing homozygous PTEN deletion with relative hemizygous loss of WAPAL and FAS signal. c Cells in the same gland showing homozygous (Homo) and hemizygous (Hemi) PTEN deletions. d PTEN deletion status among the 50 patients in the QC-TMA. e Overall quality assessment of 150 cores for FISH analysis. Intermediate quality was assigned to 53% of cores that had a detectable PTEN deletion status but also had high background to signal ratios or had areas that were over-digested. Very good quality was observed for 34% of cores that produced strong signal over low background and even digestion throughout (Additional file 2)