Fig. 4

miR-185 inhibited angiogenesis through the Nodal/ALK4 pathway. a The mRNA levels of ALK4 and VEGF were quantified by qRT-PCR in DU145 cells and LNCaP cells treated with Nodal or transfected with miR-185 mimics. b Protein levels of ALK4 and VEGF were assessed by Western blot in DU145 cells and LNCaP cells with the indicated treatments. c Representative photomicrographs of the colony formation assay and quantification of colony numbers in DU145 cells and LNCaP cells treated with Nodal or transfected with miR-185 mimics. d Cell viability of co-cultured HUVECs was evaluated by MTT assay and was determined as the percentage of the value relative to that of the control group. e The migration ability of co-cultured HUVECs was detected by transwell assay. f The tube forming ability of HUVECs was observed after the indicated treatment. The data represent one of three independent experiments. Error bars denote the mean ± SD. *P < 0.05, **P < 0.01