Fig. 4
Immuno-transcriptomic profiling reveals steady progression to immune-excluded phenotype. a Two-colour (pink and brown) DAB staining of the T cell marker CD3 (brown) and lymphocyte marker CD45 (pink) in four biopsies in tumour stroma and tumour zones. Scale bar indicates 100 µm. Quantification of b CD3+ and c CD45+ CD3- cell densities (per mm2) in each biopsy core tissue zone. Major treatments shown below. d Transcriptomic and proteomic analysis of immune cell marker expression changes over course of disease. Shaded areas indicates two-fold change threshold