Fig. 6

Inhibition of Rho/ROCK signaling blocks the functions of BMI1. a, b Protein levels of ROCK2, RhoA and RhoA-GFP (phosphorylated RhoA) in each group of cells measured by western blot analysis (*p < 0.05, two-way ANOVA); c optical density at 450 nm of each group of cells according the CCK-8 method (*p < 0.05, two-way ANOVA); d number of PI-Annexin V-double positive cells detected by flow cytometry (*p < 0.05, two-way ANOVA). Repetition = 3