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Fig. 1 | BMC Urology

Fig. 1

From: RNA-sequencing profiling analysis of pericyte-derived extracellular vesicle–mimetic nanovesicles-regulated genes in primary cultured fibroblasts from normal and Peyronie’s disease penile tunica albuginea

Fig. 1

Isolation and characterization of fibroblasts from human tunica albuginea (TA) and extracellular vesicle (EV)–mimetic nanovesicles (NVs) from mouse cavernous pericytes (MCPs). A Representative phage images (screen magnification ×40 and ×100) of primary cultured fibroblasts from the TA tissues of healthy subjects and those with PD at passage 5. B Immunofluorescent staining of fibroblasts with antibodies against CD90 (fibroblast marker), Vimentin (fibroblast marker), NG2 (pericyte marker), and CD31 (endothelial cell marker). Nuclei were labeled with the DNA dye DAPI. Scale bar indicates 100 μm. DAPI = 4,6-diamidino-2-phenylindole. C Representative phage images (screen magnification ×40) of primary cultured mouse cavernous pericytes at passage 2. D Representative western blot for EV positive markers (CD9, CD81, and TSG101) or an EV negative marker (GM130) in MCPs and pericyte-derived EV–mimetic NVs

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BMC Urology

ISSN: 1471-2490

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