Fig. 1

Differentially expressed mRNAs and miRNAs in BPS patients. A Heatmap and hieratical clustering based on normalized read counts of all mRNA (829) with p value < 0.05, absolute log2 fold change > 0.5 and mean of read counts > 50 reads. Genes are represented in y-axis and patients with BPS and controls are shown in x-axis. Normalized read counts were used for this heatmap. Minimum and maximum renormalized read counts for each mRNA were used to construct a relative color scheme by convert values to colors. One minus Pearson correlation metric was used for clustering accompanied with average linkage method. B Principal component analysis of the RNA sequencing data in a 2D graph of PC1 and PC2 based on normalized read counts of all mRNA (829) with p value < 0.05, absolute log2 fold change > 0.5 and mean of read counts > 50 reads. The bi-plot shows samples as labelled dots. Values of cos2 (square cosine, squared coordinates) for each sample indicate the quality of representation of the variables on the factor map. Samples are colour coded according to cos2 scale shown in the bar. C Volcano plot of all mRNAs. Up-regulated mRNAs are visualized in red color, and down-regulated in green. mRNAs with p value < 0.05, absolute log2 fold change > 0.5 were selected for further analysis. D Heatmap and hieratical clustering of all miRNA (62) with p value < 0.05, absolute log2 fold change > 0.5 and mean of read counts > 50 read. miRNAs are represented in y-axis and patients with BPS and controls are shown in x-axis. Normalized read counts were used for this heatmap. Minimum and maximum renormalized read counts for each miRNA were used to construct a relative color scheme by convert values to colors. One minus Pearson correlation metric was used for clustering accompanied with average linkage method. E Principal component analysis of the miRNA data in a 2D graph of PC1 and PC2. PC2 based on normalized read counts of all miRNA (62) with p value < 0.05, absolute log2 fold change > 0.5 and mean of read counts > 50 reads. The bi-plot shows samples as labelled dots. Samples are colour-coded according to the quality of representation of the variables on factor map (cos2, square cosine, squared coordinates) as shown on the side-bar. F Volcano plot of all miRNAs. All up-regulated miRNAs are visualized in red color, and all down-regulated in green. miRNAs with p value < 0.05, absolute log2 fold change > 0.5 were selected for further analysis