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Fig. 4 | BMC Urology

Fig. 4

From: Integrated mRNA-miRNA transcriptome analysis of bladder biopsies from patients with bladder pain syndrome identifies signaling alterations contributing to the disease pathogenesis

Fig. 4

QPCR validation of selected DEGs based on NGS mRNA dataset. QPCR results for 4 mRNAs, regulated and significant (p < 0.05) in NGS mRNA dataset. Gene expression was determined in RNA samples isolated from controls (n = 8) and BPS patients’ biopsies (n = 16) using TaqMan gene expression The Ct values obtained after the real time-QPCR were normalized to 18S rRNA expression and fold differences compared to the average of controls calculated. Boxplot of log2 fold changes relative to the average normalized Ct values in the control group is shown. Statistically significant differences (*p < 0.05, ***p < 0.001, ANOVA)

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BMC Urology

ISSN: 1471-2490

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