Fig. 2

The level of Glut-1 was found to be upregulated in BC tissues and negatively regulated by miR-340 overexpression. A and B The mRNA and protein expression of Glut-1 was tested in 30 pairs of BC tissues and adjacent normal tissues by RT-qPCR and western blotting, respectively. Full length blots/gels are presented in Additional file 1: Fig. S1–Additional file 2: Fig. S2. C The transfection efficiency was examined by RT-qPCR after T24 cells transfection of miR-340 mimics and miR-NC mimics for 48 h. D and E The mRNA and protein levels of Glut-1 were detected using RT-qPCR and western blotting after T24 cells transfection of miR-340 mimics and miR-NC mimics. **P < 0.01. Full length blots/gels are presented in Additional file 3: Fig. S3–Additional file 4: Fig. S4