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Fig. 1 | BMC Urology

Fig. 1

From: The routine use of LCD-Array hybridisation technique for HPV subtyping in the diagnosis of penile carcinoma compared to other methods

Fig. 1

Positive staining for anti p16 antibody leads to the assumption of HPV infection. For further validation the prerequisite is DNA extraction to elucidate HPV infection status. Workflow for Sanger sequencing (left) and LCD-Array technique (right), simplified for visualisation means: Sanger sequencing demands for a PCR, which amplifies the sequence of interrogation with specific primer sets. Thereafter single strands are subject to capillary electrophoresis technology to retrieve the sequence of interest. After editing and proving the sequence output a BLAST® search can be applied to prove the sequence of the HPV subtype. For LCD-Array technique the workflow demands for a PCR reaction which leads to biotinylated PCR products. The product can be applied on an LCD-Chip. Image analysis is automated

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BMC Urology

ISSN: 1471-2490

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