Table 2 Antibodies used for immunohistochemistry assay
Antibody | Source | Clone and manufacturer | Antigen retrieval solution pH | Dilution and incubation | Positive control |
|---|---|---|---|---|---|
Anti-p53 | Mouse monoclonal IgG1 | DO-7, Dako, Glostrup, Denmark | 10 mM Citrate buffer pH 6.0 | 1:50, overnight at 4 °C | Breast cancer sections with p53-positive nuclear staining |
Anti-p16INK4a | Mouse monoclonal IgG1 | JC8, Santa Cruz Biotechnology, California, USA | 10 mM Tris–EDTA buffer pH 9.0 | 1:50, overnight at 4 °C | Formalin-fixed HeLa cell blocks |
Anti-Ki-67 | Mouse monoclonal IgG1 | MIB-1, Dako, Glostrup, Denmark | 10 mM Citrate buffer pH 6.0 | 1:100, overnight at 4 °C | Intestine tissue sections |
Anti-NF-κB (p65) | Mouse monoclonal IgG1 | Santa Cruz Biotechnology, California, USA | 10 mM Citrate buffer pH 6.0 | 1:400, 1 h at room temperature | Formalin-fixed HeLa cell blocks |