Epstein–Barr virus infection is associated with the nuclear factor-kappa B p65 signaling pathway in renal cell carcinoma

Table 5 Semi-quantitative scoring system of NF-κB immunostaining

Percentage of immunoreactive cells	Percentage-based score	Staining intensity	Intensity-based score	Reference
0–5%	0	No staining	0	Zhou et al. [48]
6–25%	1	Straw yellow	1
26–50%	2	Brown	2
51–75%	3	Tan	3
More than 75%	4

For statistical analysis, the scoring index was calculated by addition of percentage-based scores and intensity-based scores, and the cut-off value was selected as three so that < 3 was defined as low expression and ≥ 3 was considered as high expression

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