Fig. 3

PCGEM1 directly sponged miR-506-3p. A The subcellular localization and fractionation of PCGEM1 within PC-3 and C4-2B cells were determined through qRT-PCR, with GAPDH (cytoplasmic) and U6 (nuclear) as internal references. B Binding sites in PCGEM1 versus miR-506-3p. C, D Binding sites in PC-3 and C4-2B cells were investigated by dual-luciferase assay and biotin-labeled pull-down assay. E RIP assay was performed to determine the enrichment of PCGEM1 and miR-506-3p. F MiR-506-3p mRNA expression in PC-3 and C4-2B cells with PCGEM1 knockdown detected by qRT-PCR. G miR-506-3p levels in 50 PCa cases were measured by qRT-PCR. H The relationship of miR-506-3p with PCGEM1 levels was measured through Pearson correlation analysis. ***P < 0.001