Fig. 4

200× magnification of the radical cystectomy specimen. Digital microscopy images were aquired using Olympus BX51 (microscope), Olympus UPlan Apo (objective lens), Olympus DP26 (camera), and Olympus cellSens Standard (imaging software). Hematoxylin and eosin staining exhibited the immune cells that infiltrated the tumor cells (arrow head) (a). Immunohistochemically, the immune cells were positive for PD-L1 (red arrow head), whereas the tumor cells were negative for PD-L1 (b)