Fig. 2
From: CALN1 hypomethylation as a biomarker for high-risk bladder cancer
As CpH2O did not contain enzymes, amplified Cp value could be obtained regardless of methylation. On the contrary, because CpHapII was treated with HapII, which is a methylation-sensitive restriction enzyme, the amplified Cp value of only methylated sample were obtained. In addition, as CpMspI was treated with MspI, which is a methylation-independent ristriction enzyme, cleaved Cp value can be obtained regardless of methylation. The methylation percentage was calculated using Δa obtained by subtracting CpHapII from CpH2O as an index of 2, and corrected using Δb obtained by subtracting CpMspI from control gDNA or CpH2O. CpH2O, Cp value obtained using real time PCR analysis of sample without added enzyme; CpHapII, Cp value obtained using real time PCR analysis of sample after HapII treatment; CpMspI, Cp value obtained using real time PCR analysis of sample after MspI treatment