Fig. 3

MiR-183-5p could bind to 3’-UTR of TET1. (A) Binding sites of miR-183-5p in the TET1 3’-UTR predicted by TargetScan. WT: wild type; MUT: mutated type. The mutant nucleotides are in red. (B) The direct targeting of miR-183-5p to TET1 was confirmed by dual luciferase reporter assays. (C) TET1 protein levels were analyzed using western blot in PCa and LNCaP cell lines after transfection of miR-NC and miR-183-5p mimic, respectively, and β-actin was a loading control. Western blots were cropped for clarity, uncropped images are shown in Supplementary material. (D) TET1 mRNA levels was determined by qRT-PCR in PC-3 and LNCaP cells after transfection of miR-NC and miR-183-5p mimic, respectively. Data are presented as mean ± SEM; ^****P < 0.0001; ^***P < 0.001